While taunting Spider-Man about Mary Jane in the final battle scene, Venom says, "How's that sound, tiger?" He says the same line in the 1990s Spider-Man animated series.

Stan Lee, the creator of Spider-Man, makes a cameo appearance when Peter Parker was reading about Spider-Man getting the key to the city. He is the man that walks up and talks to him.

All the screams from this movie by Kirsten Dunst were recycled from Spider-Man 2.

Director Sam Raimi's daughter makes a cameo appearance as the little girl who sells her camera to J. Jonah Jameson near the end.

Each of the black suits used in the film cost $30,000.

At the event where he is given the keys to the city, Spider-Man shouts "Shazam" as he makes his entrance. This is the catchphrase of Captain Marvel in the DC comics, the main rival to the Marvel comic book franchise.

Composer Christopher Young appears as a piano player at Mary Jane Watson's rehearsal scene.

Topher Grace is never referred to as "Venom" once in the entire movie.

Spider-Man 3 combines all three Raimi brothers. Sam Raimi as the director, Ivan Raimi as one of the writers and Ted Raimi as an actor in the role of Hoffman.